منابع مشابه
Conditional gene targeting for cancer gene therapy.
Current treatment of solid tumors is limited by severe adverse effects, resulting in a narrow therapeutic index. Therefore, cancer gene therapy has emerged as a targeted approach that would significantly reduce undesired side effects in normal tissues. This approach requires a clear understanding of the molecular biology of both the malignant clone and the biological vectors that serve as vehic...
متن کاملLessons on Conditional Gene Targeting in Mouse Adipose Tissue
Conditional gene targeting has been extensively used for in vivo analysis of gene function in adipocyte cell biology but often with debate over the tissue specificity and the efficacy of inactivation. To directly compare the specificity and efficacy of different Cre lines in mediating adipocyte specific recombination, transgenic Cre lines driven by the adipocyte protein 2 (aP2) and adiponectin ...
متن کاملConditional Gene Targeting: Dissecting the Cellular Mechanisms of Retinal Degenerations
Retinal neuron degeneration and survival are often regulated by the same trophic factors that are required for embryonic development and are usually expressed in multiple cell-types. Therefore, the conditional gene targeting approach is necessary to investigate the cell-specific function of widely expressed and developmentally regulated genes in retinal degeneration. The discussion in this revi...
متن کاملConditional Gene Targeting in Mouse Pancreatic β-Cells
OBJECTIVE Conditional gene targeting has been extensively used for in vivo analysis of gene function in β-cell biology. The objective of this study was to examine whether mouse transgenic Cre lines, used to mediate β-cell- or pancreas-specific recombination, also drive Cre expression in the brain. RESEARCH DESIGN AND METHODS Transgenic Cre lines driven by Ins1, Ins2, and Pdx1 promoters were b...
متن کاملGermline recombination by conditional gene targeting with Parvalbumin-Cre lines
Conditional gene targeting allows us to study gene function in specific tissues or cell types. This is commonly achieved by Cre DNA recombinase and its 34–base pair target sequences called loxP sites. Through the efforts of individual labs and largescale projects, a sizable collection of Cre mouse lines has been generated to express or delete specific genes in a wide range of cell types through...
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ژورنال
عنوان ژورنال: Journal of Clinical Investigation
سال: 1996
ISSN: 0021-9738
DOI: 10.1172/jci118828